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Contents and Storage
Handle under sterile conditions. Store at +4°C. Trifectin is guaranteed to be stable for a minimum of 6 months when properly stored. Do not freeze or leave at room temperature for extended periods of time.
To qualitatively assess transfection efficiency, we recommend using the dye-labeled transfection control duplexes. A variety of positive controls are available.
| Basic Protocol
Mix Trifectin with the siRNA or other nucleic acid, add to the cells, and incubate. Trifectin has low toxicity and it is not necessary to remove the transfection cocktail before an extended incubation period. Use is compatible with both forward and reverse transfection and in manual or robotic systems.
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