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OligoAnalyzer 3.1


Instructions | Definitions | Feedback  

Sequence # Bases          
Target Type  
Oligo Conc µM  
Na+ Conc  mM  
Mg++ Conc mM  
dNTPs Conc mM  
    
      
5'- -3'
  

Standard Mixed Base Instructions
To use a Standard Mixed Base, simply type in the IUB symbol
(from the table below) which represents the desired mix.
 A,G
 C,T
 A,C
 G,T
 C,G
 A,T
 A,C,T
 C,G,T
 A,C,G
 A,G,T
 A,C,G,T
Custom Mixed Base Instructions
To use Custom Mixed Bases
  • Enter the desired percentage of each base (Integers Only, Totaling 100%).
  • Press 'Use Mix Base' button to add your custom mixed base.
Please note: An additional charge is applied for hand mixing these custom bases.
 
 %A%C%G%T 
 
Base Notations
  • DNA = A, C, G, T and U, I
  • Mixed Bases = Please enter bases in UPPERCASE
  • Phosphorothioated DNA = A*, G*, C*, T*
  • RNA = rA, rG, rC, rU
  • Phosphorothioated RNA = rA*, rG*, rC*, rU*
  • 2'O-Methyl RNA = mA, mG, mC, mU
  • Phosphorothioated 2'O-Methyl RNA = mA*, mG*, mC*, mU*
  • Locked Nucleic Acid (LNA) = +A, +G, +C, +T
 
 
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