Integrated DNA Technologies

ReadyMade™ Randomers

Randomers are oligonucleotides composed of all possible sequences for a given length. The use of randomers began in the early 1980s when random hexamers were employed in radio labeling DNA probes^1,2. A later application was the use of random sequence primers to detect Random Amplified Polymorphisms (RAPDs)^3,4.

Today, random sequence oligonucleotides are being used to detect Single Nucleotide Polymorphisms (SNPs) as well as small scale chromosome events, primarily insertions or deletions^5,6. Comparative Genomic Hybridization (CGH) has been developed to elucidate genome-wide sequence copy-number variation (CNV) between different genomes, such as the differential amplification or deletion of genetic regions between tumor DNA and normal DNA from neighboring unaffected tissue^7-10. These applications demand that the oligonucleotides have consistent base composition and minimal lot-to lot variation.

IDT Randomers are made using an N-base phosphoramidite mixture that is carefully prepared in bulk to ensure that equal base representation is achieved. The batch is then tested by making test randomer oligos and checking actual base composition by enzymatic degradation and HPLC.

ReadyMade Random Hexamers

Description	Price
10 nm 5' Biotinylated Random Hexamer	$27.50 USD
10 nm Random Hexamer	$25.00 USD
50 nm 5' Biotinylated Random Hexamer	$112.50 USD
50 nm Random Hexamer	$100.00 USD
500 nm 5' Biotinylated Random Hexamer	$875.00 USD
500 nm Random Hexamer	$775.00 USD

ReadyMade Random Nonamers

Description	Price
10 nm 5' Cy3 Random Nonamer	$47.50 USD
10 nm 5' Cy5 Random Nonamer	$47.50 USD
10 nm 5' TYE 563 Random Nonamer	$42.75 USD
10 nm 5' TYE 665 Random Nonamer	$42.75 USD
50 nm 5' Cy3 Random Nonamer	$150.00 USD
50 nm 5' Cy5 Random Nonamer	$150.00 USD
50 nm 5' TYE 563 Random Nonamer	$135.00 USD
50 nm 5' TYE 665 Random Nonamer	$135.00 USD
500 nm 5' Cy3 Random Nonamer	$875.00 USD
500 nm 5' Cy5 Random Nonamer	$875.00 USD
500 nm 5' TYE 563 Random Nonamer	$795.00 USD
500 nm 5' TYE 665 Random Nonamer	$795.00 USD

ReadyMade Randomers Nonamer Matched Sets for CGH

Description	Price
10 nm 5' Cy3 & 5' Cy5 Random Nonamer Set	$85.00 USD
10 nm 5' TYE 563 & 5' TYE 665 Random Nonamer Set	$77.50 USD
50 nm 5' Cy3 & 5' Cy5 Random Nonamer Set	$275.00 USD
50 nm 5' TYE 563 & 5' TYE 665 Random Nonamer Set	$275.00 USD
500 nm 5' Cy3 & 5' Cy5 Random Nonamer Set	$1,575.00 USD
500 nm 5' TYE 563 & 5' TYE 665 Random Nonamer Set	$1,425.00 USD

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References

1. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Feinberg, A.P., and Vogelstein, B. Analytical Biochemistry, 132: 6-13 (1983).
2. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Feinberg, A.P., and Vogelstein, B. Analytical Biochemistry, 137: 266-7 (1984).
3. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, A.J., and Tingey, S.V. Nucleic Acids Research 18: 6531-6535 (1990).
4. Forrest, A. K. et al. (2000). Aminoalkyl adenylate and aminoacyl sulfamate intermediate analogues differing greatly in affinity for their cognate Staphylococcus aureus aminoacyl tRNA synthetases. Bioorg. Med. Chem. Letters 10, 1871-1874.
5. Comparative Genomic Hybridization: an Overview. Houldsworth, J., and Chaganti, R. American Journal of Pathology 145: 1253-1260 (1994).
6. Detection of chromosomal aberrations by means of molecular cytogenetics: painting of chromosomes and chromosomal subregions and comparative genomic hybridization.
7. Comparative Genomic Hybridization for Molecular Cytogenetic Analysis of Solid Tumors. Kallioniemi, A., Kallioniemi, O-P., Sudar, D., Rutovitz, D., Gray, J.W., Waldman, F., and Pinkel, D. Science 258:818-821 (1992).
8. High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Pinkel, D., Segraves, R., Sudar, D., Clark, S., Poole, I., Kowbel, D., Collins, C., Kuo, W-L., Chen, C., Zhai, Y. et al. Nature Genetics 20:207-211 (1998).
9. From microscopes to microarrays: dissecting recurrent chromosomal rearrangements. Emanuel, B.S., and Saitta, S.C. Nature Reviews Genetics 8(11):869-83 (2007).
10. Comparing whole genomes using DNA microarrays. Gresham, D., Dunham, M.J., and Botstein, D. Nature Reviews Genetics 9(4):291-302 (2008).