Validated Control DsiRNA Duplexes and other Control Reagents
| The following collection of validated control reagents are available premade for immediate delivery. Inquire for custom controls for special needs.
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A successful RNAi experiment starts with good transfection. It is good practice to optimize transfection conditions for each cell line studied as well as for each form of nucleic acid employed (for example, large DNA plasmids often require different transfection conditions than short dsRNA oligos). It may be necessary to empirically test a number of different cationic lipids (or other approaches) to establish a protocol that performs optimally with each cell line employed. Use of a dye-labeled transfection control oligo allows for rapid, easy screening of many reagents in parallel.
When optimizing transfection methods, IDT recommends using dye-labeled oligos at 10 nM (or less); higher concentrations can increase the amount of non-specific binding which can cause background and falsely elevate the apparent success of transfection.
The following dye-labeled RNA duplexes are available as ReadyMade products for this purpose. Other dyes can be provided on RNA oligos on a custom basis. For example, the LI-COR IRdye® 800 CW can be placed on RNA duplexes and employed for in vivo biofluorescence imaging, with excellent tissue penetration due to the far infrared spectral emission of this fluorophore.
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