DNA & RNA

Thevalueofknowingyourprimerandprobesequences
Demonstratingfeaturesoftheoligoentrytool

Genome editing

IncreasinggenomeeditingefficiencywithoptimizedCRISPR-Casenzymes
OptimizedmethodstouseCas9nickasesingenomeediting
HowtoorderAlt-RCRISPRcrRNAinplates

Next generation sequencing

BestpracticesfordataanalysiswhenusingUMIadapterstoimprovevariantdetection
Thequestforhighconfidencemutationsinplasmasearchingforaneedleinahaystack
DualindexadapterswithUMIsresolveindexhoppingandincreasesensitivityofvariantdetection

Genes & gene fragments

IDTSyntheticBiologyAdvantage
TipsforworkingwithgBlockssupregsupGeneFragments
RapidandreliablegeneconstructionwithgBlocksGeneFragmentsandtheGibsonAssemblyMethod

qPCR & PCR

SNPgenotypingonqPCRplatformsTroubleshootingforamplificationandclusterseparation
rhAmpSNPgenotypingAnovelapproachforimprovingPCR-basedSNPgenotyping
High-throughputqPCRTipsforanalysisacrossmultipleplates

Functional genomics

KnockdownoflncRNAsExploringRNAiandantisenseoligomethods
DsiRNADesignTool
PlanningandexecutingsiRNAexperiments

General molecular biology

ProducingpeptidesusingbacteriaAnalternativeapproach
UseofNCBIdatabasesinqPCRassaydesign
Single-cellexpressionprofiling