DNA & RNA

Thevalueofknowingyourprimerandprobesequences
Demonstratingfeaturesoftheoligoentrytool

Genome editing

ACRISPRQAwithourexpertsYouaskweanswer
IncreasinggenomeeditingefficiencyandspecificitywithoptimizedCRISPR-Cas9guideRNAs
OvercomingthechallengesofdesigningefficientandspecificCRISPRgRNAs

Next generation sequencing

BestpracticesfordataanalysiswhenusingUMIadapterstoimprovevariantdetection
Thequestforhighconfidencemutationsinplasmasearchingforaneedleinahaystack
DualindexadapterswithUMIsresolveindexhoppingandincreasesensitivityofvariantdetection

Genes & gene fragments

TipsforworkingwithgBlockssupregsupGeneFragments
IDTSyntheticBiologyAdvantage
RapidandreliablegeneconstructionwithgBlocksGeneFragmentsandtheGibsonAssemblyMethod

qPCR & PCR

DesignconsiderationsforqPCRassays
SNPgenotypingonqPCRplatformsTroubleshootingforamplificationandclusterseparation
rhAmpSNPgenotypingAnovelapproachforimprovingPCR-basedSNPgenotyping

Functional genomics

KnockdownoflncRNAsExploringRNAiandantisenseoligomethods
DsiRNADesignTool
PlanningandexecutingsiRNAexperiments

General molecular biology

ProducingpeptidesusingbacteriaAnalternativeapproach
UseofNCBIdatabasesinqPCRassaydesign
Single-cellexpressionprofiling