DNA origami self-assembly requires 2 types of DNA, a scaffold strand and a mixture of staple strands. A scaffold strand is a long piece of single-stranded DNA. Staple strands are single-stranded 30–80 nt DNA oligos that direct the sequence-specific folding of the scaffold strand into the desired configuration.
The single-stranded Scaffold DNAs are produced by tilibit nanosystems and are available in 3 lengths—7249, 7560, and 8064 bases—either individually or as part of a convenient Folding Kit. The tilibit nanosystems Folding Kits contain Scaffold DNA, Folding Reaction Buffer, and an EDTA-free Loading Dye, custom developed to support straight bands in agarose gel electrophoresis. Staple Strand Oligos are synthesized by IDT.
Because DNA origami design is complex, we recommend that new users contact the tilibit nanosystems DNA origami experts for design assistance prior to ordering DNA origami reagents, including the reagents required to build the predesigned structures described below (Table 1).
Predesigned DNA origami
Tilibit nanosystems offers 6 standard DNA origami structures. See Table 1 and www.tilibit.com/collections/prefabricated-structures for details. Contact tilibit nanosystems to determine whether one of these predesigned structures fits your research needs or whether you will need a custom design. Note: To minimize aggregation, all structures are passivated by including poly-T sequences or single-stranded scaffold loops as described in Table 1.