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ReadyMade™ Primers

HPLC purified, inventoried oligonucleotides for sample preparation, sequencing, and gene expression analysis of common genes.

Ordering

ReadyMade Primers include random hexamers, T7 promoter/terminator, M13 primers, 16S rRNA gene primers, and varieties of oligo dT that are available for same-day shipping.

  • Routine purity >90%
  • Identity confirmed via ESI-mass spectrometry*
  • Obtain 10 µg of dry product—enough for 100 sequencing reactions

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Product details

ReadyMade Primers are stocked oligonucleotides for sample preparation, PCR, sequencing, and gene expression analysis of common genes. Each primer contains 10 μg of HPLC-purified product. Identity is confirmed by mass spectrometry* and purity is established by capillary electrophoresis. Because these primers are inventoried, they can be shipped as soon as your order is received. Examples of ReadyMade Primers include random hexamers, T7 promoter/terminator, M13 primers, 16S rRNA primers, and varieties of Oligo dT. See the product list for our full catalog of ReadyMade Primers and their specific properties.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

PrimerSequence Tm (qPCR conditions)Spec Sheet (50nM Na+)Anhydrous MWpmol in
10 µg
16S rRNA For AGA GTT TGA TCC TGG CTC AG 61.354.36148.0 1626.7
16S rRNA Rev ACG GCT ACC TTG TTA CGA CTT 63.5 56.1 6372.2 1569.4
3' RACE PCR GGC CAC GCG TCG ACT AGT AC 66 60.1 6103.0 1638.5
Anchored Oligo dT (20) TTT TTT TTT TTT TTT TTT TV 48 37.5 6028.3 1658.7
Anchored Oligo dT (22) TTT TTT TTT TTT TTT TTT TTV N 51.5 41 6641.4 1505.7
BGH Reverse TAG AAG GCA CAG TCG AGG 60.2 53.6 5597.7 1786.4
Bluescript KS TCG AGG TCG ACG GTA TC 59.5 53.2 5226.4 1913.4
cDNA Cloning Primer GGC CAC GCG TCG ACT AGT ACT TTT TTT TTT TTT TTT TV 70.2 61.5 11584.9 863.1
EGFP-C CAT GGT CCT GCT GGA GTT CGT G 66.6 60.2 6773.4 1476.3
EGFP-N CGT CGC CGT CCA GCT CGA CCA G 71.7 66.4 6657.3 1502.1
GAPDH For ACC ACA GTC CAT GCC ATC AC 64.5 57.7 5991.0 1669.3
GAPDH Rev TCC ACC ACC CTG TTG CTG TA 65.4 58.6 6003.9 1665.7
M13 Forward (-20) GTA AAA CGA CGG CCA GT 59.4 52.6 5228.5 1912.6
M13 Forward (-41) CGC CAG GGT TTT CCC AGT CAC GAC 70.3 64.1 7289.8 1371.7
M13 Reverse (-27) CAG GAA ACA GCT ATG AC 54.2 47 5212.5 1918.3
M13 Reverse (-48) AGC GGA TAA CAA TTT CAC ACA GG 63.4 55.7 7065.7 1415.2
Neomycin For CTT GGG TGG AGA GGC TAT TC 61.6 54.9 6204.1 1612.0
Neomycin Rev AGG TGA GAT GAC AGG AGA TC 60.3 53.2 6255.1 1598.7
Oligo dT, 15mer TTT TTT TTT TTT TTT 38.6 28.4 4501.0 2221.7
Oligo dT, 16mer TTT TTT TTT TTT TTT T 40.9 30.64805.2 2081.0
Oligo dT, 18mer TTT TTT TTT TTT TTT TTT 44.8 34.3 5413.6 1847.3
Oligo dT, 20mer TTT TTT TTT TTT TTT TTT TT 48 37.3 6022.0 1660.6
Oligo dT, 20mer w/ 5' Phos /5Phos/TTT TTT TTT TTT TTT TTT TT 48 37.3 6101.9 1638.8
PCMV Forward CGC AAA TGG GCG GTA GGC GTG 69.8 63.9 6552.3 1526.2
pET 3' CTA GTT ATT GCT CAG CGG 57.1 50.2 5505.6 1816.2
pET 5' (T7) TAA TAC GAC TCA CTA TAG G 52.4 44.5 5795.8 1725.3
pGEX 3' CCG GGA GCT GCA TGT GTC AGA GG 70 64 7145.7 1399.3
pGEX 5' GGG CTG GCA AGC CAC GTT TGG TG 71.8 65.7 7136.6 1401.1
Random Hexamer NNN NNN <10 <10 1791.7 5581.3
Random Hexamer w/ Biotin /5Bio/NNN NNN <10 <10 2198.2 4549.3
SP6 Promoter TAC GAT TTA GGT GAC ACT ATA G 56.9 48.8 6773.5 1476.2
SP6 Upstream ATT TAG GTG ACA CTA TAG 50.2 42.1 5537.7 1805.8
T3 Promoter AAT TAA CCC TCA CTA AAG GG 57.1 49.3 6094.0 1640.9
T7 Promoter TAA TAC GAC TCA CTA TAG GG 55.2 47.5 6125.0 1632.5
T7 Terminator GCT AGT TAT TGC TCA GCG G 60.3 53.4 5834.8 1713.8
RUO22-1228_002