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rhAmp™ SNP Genotyping System

Accurate, affordable genotyping with the next evolution of PCR

Improve the precision of your PCR-based SNP genotyping with rhAmp SNP Genotyping technology. This technology uses a unique two-enzyme system coupled with RNA-DNA hybrid primers to precisely interrogate target SNPs. Combined with IDT universal reporter chemistry, the rhAmp SNP Genotyping System offers a simple, high performance genotyping solution at an affordable price.

  • Generate the highest level of performance with greater than 99.5% call accuracy for over 90% of assays tested
  • Interrogate SNPs in difficult sequence regions with amplicons sizes as small as 40 bp
  • Validate markers affordably using the smallest pack size commercially available
  • Ensure confidence in your data with gBlocks® Gene Fragments as control templates

The rhAmp SNP portfolio includes all components needed to successfully generate high quality genotyping data on any commonly available real-time PCR instrument.


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rhAmp SNP Assays


The predesigned rhAmp SNP Assay collection contains assays for over 10 million human SNPs, including a broad selection of functionally validated rhAmp ADME SNP assays.

An online, custom assay design tool is also available for newly discovered human SNPs or assay designs for other species.

Note: Synthetic control templates are available during an integrated ordering process.

Learn more about rhAmp SNP Assays »

rhAmp Genotyping Master Mix and Reporter Mixes

rhAmp Genotyping Master Mix and rhAmp Reporter Mixes are specially formulated for use with rhAmp SNP Assays to deliver superior specificity and signal generation.

Learn more about rhAmp reagent mixes »


Fast and simple reaction setup

A single-tube assay setup allows for routine automation and delivers genotypes with only 90 minutes of cycling time (Figure 1). Hot-start enzymes enable benchtop reaction setup and support reactions that are stable for up to 2 days before and after cycling at room temperature.

Genotyping workflow

Figure 1. Simple, one-tube reaction chemistry supports streamlined lab processes. All reagents are combined in the initial reaction setup that is stable for up to 24 hours at room temperature before cycling. rhAmp™ SNP Genotyping is compatible with common qPCR platforms.

Superior chemistry for SNP detection

rhAmp SNP Genotyping uses novel, blocked primers to minimize primer dimer artifacts and other non-specific amplification. A single RNA base is incorporated into rhAmp primers, and that RNA base is cleaved by RNase H2 enzyme only if it is hybridized to its perfect complement, activating the primers for extension. This target-specific, RNase-H2 activation increases the specificity of rhAmp SNP Assays.

A novel Taq DNA Polymerase (IDT) with enhanced sensitivity for allelic mismatches provides extension from the deblocked primers, and degrades the reporter probes (Figure 2).


 


rhAmp SNP Gentotyping reaction

Figure 2. Schematic representation of a rhAmp™ SNP Genotyping PCR cycle. All components needed to measure both alleles are combined in a single reaction before cycling. 1) Both allele-specific primers query the SNP locus. 2) RNase H2 enzyme cleaves the primers that are perfectly annealed to the target sequence, removing the RNA base and 3′ blocking modification, which allows extension by the IDT Taq Polymerase. 3) During the first two amplification cycles, a tail sequence is incorporated into the amplicon that is subsequently recognized by a universal, probe-based reporter system. 4) Polymerase extension leads to degradation of the probe and signal generation.