Figure 1. Optimal performance of xGen® Lockdown® Probes and Panels using xGen Lockdown Reagents. Target enrichment was performed using a DNA library that was created from human genomic DNA (Coriell) and enriched for a 57 kb region of the human genome. The hybridization and wash steps were performed using xGen Lockdown Reagents (IDT) or the SeqCap® EZ Hybridization and Wash Kit (NimbleGen). The enriched library was sequenced on an Illumina MiSeq® system. The data show (A) greater depth of coverage and (B) better coverage uniformity with xGen Lockdown Reagents.