IDT SciTools® Web Tools These software applications determine the properties of any oligo sequence entered, as well as facilitate the intelligent design of assay conditions, all at the click of a button. The suite is easy to use and makes it simple to add each optimized oligonucleotide to an ongoing order list ready for purchase. We Value Your Input! Help us improve your website experience by participating in a quick online exercise and then answering a few questions.Take the survey » Oligo Analysis Tools OligoAnalyzer® Tool Identifies oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches UNAFold Tool Secondary structure analysis Dilution Calculator Find the volume needed to dilute your oligo stock to a lower concentration Resuspension Calculator Find the volume needed to resuspend a dry oligo to a desired concentration qPCR and PCR Amplification PrimeTime® Predesigned qPCR Assay Database Selects qPCR primers and probe sequences for human, mouse, and rat assays based on up-to-date sequence data PrimerQuest® Tool Allows highly customized designs for PCR primers and qPCR assays RealTime PCR Tool Selects custom qPCR assays for any species Gene Regulation and Knockdown Predesigned DsiRNA Selection Tool Selects DsiRNA Duplexes and TriFECTa® Screening Kits for your sequence RNAi Design Tool Generates duplex siRNA sequences for RNAi applications Gene Design Codon Optimization Tool Automates codon optimization of a gene to the species of your choice Sequencing and miRNA 454 Fusion Primers Designs primers for next generation sequencing on 454 GS GLX Standard or Titanium Platforms miRNA Detection Kit Selection Tool Identifies the IDT mirFire™ Probe or Labeling Kit needed to label a specific miRNA Please use following format when citing SciTools: PrimerQuest® program, IDT, Coralville, USA. Retrieved 12 December, 2012. http://www.idtdna.com/Scitools.