- Recommended products—curated for whole genome sequencing
- Short workflow—create a normalized library pool in approximately 4 hours
- Low input, high complexity—up to 3x’s fewer duplicates from 1 ng of DNA
- Enzymatic fragmentation—no need for specialized equipment for library preparation
- xGen Normalase™ technology—a proprietary enzymatic method of library normalization for multiplexing applications
- Automation friendly—for a variety of platforms
Whole genome sequencing provides a comprehensive set of genomic sequence
data about an organism, tissue, or even metagenomic DNA samples. In comparison to targeted sequencing,
WGS decodes each of the fragments in the NGS indexed libraries. The sequence data is then aligned to a reference genome or assembled into contigs for de novo genome assemblies.
For low-throughput labs, the 16-reaction product solution for whole genome sequencing includes library prep and adapters. For library prep, the xGen DNA Library Prep Kit EZ uses an enzymatic fragmentation strategy to shear high-quality DNA samples. The kit employs ligation-based library prep, and it includes enzymes for fragmentation, end-repair, a high-fidelity polymerase for indexing PCR, and A‑tailing, as well as a ligase
for the addition of the xGen Stubby Adapters (included). The product solution offers premixed xGen UDI Primer Pairs for indexing by PCR, which generates a NGS library ready for Illumina® sequencing.
For high-throughput labs, the 96-reaction size includes the xGen Normalase Module,
a proprietary enzymatic library normalization kit for multiplexing. The method eliminates the need for individual sample quantification followed by manual equimolar pooling. Instead, the fragments are indexed by PCR using the included plate of xGen
Normalase UDI Primer Pairs followed by the xGen Normalase Module. Final normalized library pools are then ready for Illumina®-based multiplex sequencing.