Producing peptides using bacteria: An alternative approach

While chemically synthesized peptides are excellent tools, the in-house production of peptides using bacteria offers clear advantages and great flexibility regarding mutational analysis and the use of additional (removable) tags to: promote cellular uptake; to perform affinity purifications; and to analyze via Western-blotting analysis and immunocytochemistry. In this webinar we emphasize our work with peptides and present an alternative strategy to generating peptides using custom MiniGene synthesis from IDT. We share our experiences with this system, providing a balanced opinion towards this alternative method with its advantages and disadvantages. In particular, We provide more details about our endeavors to produce purified BH4 domain of Bcl-2 (and mutant forms of this peptides) using recombinant expression in E. coli by MiniGene synthesis.

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